Key Features and Values

– Same sample type can be used across all assays to simplify inclusion into routine serology work-up
– Ready to use reagents reduces hands-on time for assay preparation (1 lyophilised reagent)
– Long shelf life cost-effective solution by reducing wastage due to expired kits
– Suitable for inclusion on automated plate systems simplifies scale-up of test volume
– Supported by a complete panel of assays for supporting treatment monitoring of several forms of cancer

Product Description

Immunoenzymatic colorimetric method for the quantitative determination of hNSE ELISA concentration in human serum. hNSE ELISA kit is intended for laboratory use only.

Scientific Description
Neuron Specific Enolase (2-phospho-D-glycerate hydrolase) is an isoenzyme that belongs to the enolase family (homo- and heterodimer constituted of α, β and γ subunit) that is distinguished from these by the presence of the specific γγ heterodimer.
The clinical usefulness of hNSE like tumour marker is compared to non small cell lung cancer (NSCLC), to neuroblastoma, to medullary carcinoma of the thyroid, pancreatic islet cell tumour and to non neoplastic condition of neuronal disease and cerebral trauma.
hNSE ELISA test cannot be used as a screening test for neuroendocrine tumours, but may be used to follow levels in established diagnosis.

1.Sorensen K, Brodbeck U, Paus E, Norgaard-Pedersen B. An enzyme antigen immunoassay for the determination of neuron-specific enolase in serum samples. Clin Chim Acta. 1988 Jul 29;175(3):337-43.
2. Drivsholm L, Osterlind K, Cooper EH, Purves DA.  Neuron-specific enolase (NSE) in serum. Comparison of monoclonal versus polyclonal assay based on 392 blood samples. Int J Biol Markers. 1995 Jan-Mar;10(1):1-4.
3. Karnak D, Beder S, Kayacan O, Ibis E, Oflaz G. Neuron-specific enolase and lung cancer. Am J Clin Oncol. 2005 Dec;28(6):586-90.
4. Berger RP, Dulani T, Adelson PD, Leventhal JM, Richichi R, Kochanek PM. Identification of inflicted traumatic brain injury in well-appearing infants using serum and cerebrospinal markers: a possible screening tool. Pediatrics. 2006 Feb;117(2):325-32.
5. Ghayumi SM, Mehrabi S, Doroudchi M, Ghaderi A. Diagnostic value of tumor markers for differentiating malignant and benign pleural effusions of Iranian patients. Pathol Oncol Res. 2005;11(4):236-41. Epub 2005 Dec 31.
6. Sawauchi S, Taya K, Murakami S, Ishi T, Ohtsuka T, Kato N, Kaku S, Tanaka T, Morooka S, Yuhki K, Urashima M, Abe T. Serum S-100B protein and neuron-specific enolase after traumatic brain injury. No Shinkei Geka. 2005 Nov;33(11):1073-80
7. Ramont L, Thoannes H, Volondat A, Chastang F, Millet MC, Maquart FX. Effects of hemolysis and storage condition on neuron-specific enolase (NSE) in cerebrospinal fluid and serum: implications in clinical practice. Clin Chem Lab Med. 2005;43(11):1215-7.


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Code: DKO073
Clinical Area:
Incubation: 60+15 min
Sensitivity: N/A
Specificity: N/A
Classification: IVD, CE
Number of Tests: 96
Sample Type: Serum
Sample Volume: 25 μL
Assay Range: 4 - 100 ng/mL