Key Features and Values
– Unique mAbs developed against specific linear epitopes
– Highly sensitive
– Specific to human AMH (associated form)
– Complementary AMH products available
– Automation ready
Product Description
The Ultra-Sensitive Anti-Müllerian hormone/ Müllerian inhibiting substance (US AMH/MIS) enzyme linked immunuosorbent assay (ELISA) kit provides materials for the quantitative measurement of AMH/MIS in human serum, plasma and other biological fluids. This assay is intended for in vitro diagnostic use only.
Scientific Description
Anti-Müllerian hormone (AMH), a member of the TGFβ superfamily, is a homodimeric glycoprotein composed of two 55 kDa N-terminal and two 12.5 kDa C-terminal homodimers, non-covalently linked by disulfide bridges.
Recent studies have shown that the AMH C-terminal homodimer is much less active than the noncovalent complex, but almost all activity can be restored by associating with the N-terminal pro-region, which reforms a complex with the mature C-terminal homodimer. This finding raises the possibility that the AMH noncovalent complex is the active form of protein. It was reported that the cleaved AMH noncovalent complex binds to AMHRII and stimulates intracellular signaling, whereas full-length AMH shows only minimal activity1.
AMH is secreted by the Sertoli cells in males. During embryonic development, AMH is responsible for Müllerian duct regression. AMH continues to be produced by the testes until puberty and then decreases slowly to residual post-puberty values. In females, AMH is produced by the granulosa cells of small growing follicles from the 36th week of gestation onwards until menopause when levels become undetectable. Potential clinical applications of low end anti-müllerian hormone (AMH) have been published in premature ovarian insufficiency, ovarian tumors, menopause and many more.
Publications
1. di Clemente et al. Mol Endocrinol, November 2010, 24 (11): 2193-2206.