Androstenedione Saliva ELISA

Key Features and Values

– Same sample type can be used across all assays to simplify inclusion into routine serology work-up
– Ready to use reagents reduces hands-on time for assay preparation
– Long shelf life provides a cost-effective solution by reducing wastage due to expired kits
– Suitable for inclusion on automated plate systems simplifies scale-up of test volume
– Supported by a complete panel of assays for supporting treatment monitoring of several forms of hormonal dysfunctions
– Suitable for use in a wide range of areas including sports medicine, paediatrics, occupational health, veterinary medicine, sleep disturbance, stress monitoring and hormone replacement therapy

Product Description

Competitive immunoenzymatic colorimetric method for the quantitative determination of Androstenedione concentration in saliva. Androstenedione Saliva ELISA kit is intended for laboratory use only.

Scientific Description
Androstenedione (also known as Δ4-androstenedione) is a steroid hormone produced in the adrenal glands and the gonads as an intermediate step in the biochemical pathway that produces the androgen testosterone and the estrogens estrone and oestradiol.  It is the common precursor of male and female sex hormones.  Some androstenedione is also secreted into the plasma and may be converted in peripheral tissues to testosterone and estrogens.
Androstenedione has relatively weak androgenic activity, estimated at ~ 20% of testosterone.  Secretion and production rates also exceed those of testosterone in women in whom significant extra-adrenal conversion of androstenedione to testosterone occurs.
In premenopausal women the adrenal glands and ovaries each produce about half of the total androstendione (about 3 mg/day).  After menopause androstenedione production is about halved, primarily due to the reduction of steroid secreted by the ovary.  Nevertheless, androstenedione is the principal steroid produced by the postmenopausal ovary.
The high serum-saliva correlation for androstenedione suggests that individual differences in serum androstenedione levels may be accurately estimated using saliva as a non-invasive alternative specimen.
Publications

1. Judd H. and Yen S. J.Clin.Endoc.& Metab.,36 475 (1973)
2. Abraham G. J. Clin.Endoc. &M.39, 340 (1974)
3. Hillier S.G. 79th Year book Medical Publishers Inc: Chicago. (1985)
4. Venturoli S. et al Fertility and Sterility, 48(1), 78 (1987)
5. Venturoli S. et al Hormone Res., 24, 269 (1986)
6. D. Riad et al Endocr. Reviews, 3 (4) 304 367 (1982)

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Code: DKO027
Clinical Area:
Incubation: 60+15 min
Sensitivity: N/A
Specificity: N/A
Classification: RUO
Number of Tests: 96
Sample Type: Saliva
Sample Volume: 50 µL
Assay Range: 5 - 1000 pg/mL