Testosterone Saliva ELISA

Key Features and Values

– Same sample type can be used across all assays to simplify inclusion into routine serology work-up
– Ready to use reagents reduces hands-on time for assay preparation
– Long shelf life provides a cost-effective solution by reducing wastage due to expired kits
– Suitable for inclusion on automated plate systems simplifies scale-up of test volume
– Supported by a complete panel of assays for supporting treatment monitoring of several forms of hormonal dysfunctions
– Suitable for use in a wide range of areas including sports medicine, paediatrics, occupational health, veterinary medicine, sleep disturbance, stress monitoring and hormone replacement therapy

Product Description

Competitive immunoenzymatic colorimetric method for quantitative determination of Testosterone concentration in saliva. Testosterone Saliva ELISA kit is intended for laboratory use only.

Scientific Description
Testosterone (17β-Hydroxy-4-androstene-3-one) is a steroid hormone from the androgen group.  In postpubertal males, testosterone is secreted primarily by the testes with only a small amount derived from peripheral conversion of androstenedione.  In adult women over 50% of serum testosterone is derived from peripheral conversion of androstenedione secreted by the adrenal and ovary, with the remainder from direct secretion of testosterone by these glands.  The level of testosterone in saliva (pg/mL) is significantly lower than levels in the general circulation (ng/mL).
Testosterone effects can be classified as virilising and anabolic effects, although the distinction is somewhat artificial, as many of the effects can be considered both.  Anabolic effects include growth of muscle mass and strength, increased bone density and strength, and stimulation of linear growth and bone maturation.  Virilising effects include maturation of the sex organs, and after birth (usually at puberty) a deepening of the voice, growth of the beard and axillary hair (male secondary sex characteristics).
Testosterone levels decline gradually with age in men (andropause).  The signs and symptoms are nonspecific and are generally associated with aging such as loss of muscle mass and bone density, decreased physical endurance, decreased memory ability and loss of libido.  In females of all ages, elevated testosterone levels can be associated with a variety of virilising conditions, including adrenal tumours and polycystic ovarian disease.

1. Joshi, U. M., et al. Steroids 34 (1) 35 (1979)
2. Turkes, A., et al. J Endocrinol. 81 (2) P165 (1979)
3. Ismail A.A, et al J. Clin. Endocr. Metab. 34, 177-184 (1972)
4. Rajkowski K.M, et al Steroids 29 (5) 1977
5. Widsdom G. B., Clin.Chem. 22/8, 1243-1255(1976)


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Code: DKO021
Clinical Area:
Incubation: 60+15 min
Analytical range: 10-1000 pg/mL
Sensitivity: 3,28 pg/mL
Classification: IVD, CE
Number of Tests: 96
Sample Type: Saliva
Sample Volume: 100 μL
Assay Range: 10-1000 pg/mL