Insulin ELISA

Key Features and Values

– High sensitivity assays provide accurate measurements leading to improved performance in clinical use
– Same sample type can be used across all assays to simplify inclusion into routine serology work-up and provide a cost effective solution
– Long shelf life cost-effective solution by reducing wastage due to expired kits
– Suitable for inclusion on automated plate systems simplifies scale-up of test volume
– Supported by a panel of assays for supporting early detection and differential diagnosis of diabetes type I and II

Product Description

Insulin ELISA kit is a direct solid phase enzyme immunoassay for the quantitative determination of insulin in human serum or plasma.  Insulin ELISA kit is intended for laboratory use only.

Scientific Description

Insulin is a polypeptide hormone that regulates carbohydrate metabolism. Apart from being the primary effector in carbohydrate homeostasis, it has effects on fat metabolism and it can change the liver’s ability to release fat stores. Insulin is involved in: control of cellular intake of glucose in muscle and adipose tissue, increase of DNA replication and protein synthesis, modification of the activity of numerous enzyme (allosteric effect), increased glycogen, fatty acid synthesis, amino acid uptake, decreased proteinolysis, lipolysis and gluconeogenesis. Beta cells release insulin in a glucose-dependent way. In most humans blood glucose levels vary from about 70 mg/dL to perhaps 110 mg/dL (3.9 to 6.1 mmol/L) except shortly after eating when the blood glucose level rises temporarily. This homeostatic effect is the result of many factors, of which hormone regulation is the most important.
There are several conditions in which insulin disturbance is pathologic: diabetes mellitus, insulinoma, metabolic syndrome and polycystic ovary syndrome. There are two types of diabetes mellitus: type 1 (autoimmune-mediated destruction of insulin producing beta cells in the pancreas resulting in absolute insulin deficiency) and type 2 (multifactor syndrome with combined influence of genetic susceptibility and influence of environmental factors, the best known being obesity, age, and physical inactivity, resulting in insulin resistance in cells requiring insulin for glucose absorption. This form of diabetes is strongly inherited). In both cases the insulin production must be increased by medication or delivering insulin by intravenous method. The quantitative determination of insulin can help to determinate the dose to delivery.

Complementary Products


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Code: DKO076
Clinical Area:
Incubation: 120+15 min
Sensitivity: N/A
Specificity: N/A
Classification: IVD, CE
Number of Tests: 96
Sample Type: Serum/Plasma
Sample Volume: 100 μL
Assay Range: 3-200 µIU/mL