LH ELISA

Key Features and Values
  • Same sample type can be used across all assays to simplify inclusion into routine serology work-up
  • Ready to use reagents reduces hands-on time for assay preparation
  • Long shelf life cost-effective solution by reducing wastage due to expired kits
  • Suitable for inclusion on automated plate systems simplifies scale-up of test volume
  • Supported by a complete panel of assays for supporting treatment monitoring of several forms of hormonal dysfunctions
Product Description

LH ELISA kit is a solid phase enzyme immunoassay for the quantitative determination of luteinising hormone (LH) concentration in human serum or plasma.  The LH ELISA kit is intended for laboratory use only.

Scientific Description

Luteinising hormone (LH) is a glycoprotein consisting of two subunits with a molecular mass of 30,000 daltons.  The α-subunit is similar to other pituitary hormones [follicle stimulating hormone (FSH), thyroid stimulating hormone (TSH) and chorionic gonadotropin (HCG)] while the β-subunit is unique.  The β-subunit confers the biological activity to the molecule.  The α-subunit consists of 89 amino acid residues while the β-subunit contains 129 amino acids. The carbohydrate content is between 15% and 30%.  The clinical usefulness of the measurement of LH in ascertaining the homeostasis of fertility regulation via the hypothalamic – pituitary – gonadal axis has been well established1,2.  In addition, the advent of in vitro fertilisation (IVF) technology to overcome infertility associated problems has provided the impetus for rapid improvement in LH assay methodology from the technically demanding bioassay3 to the procedurally simple and rapid  immunoenzymometric assays.

Publications

1. Kosasa T.S., “Measurement of Human Luteinizing Hormone.” Journal of Reproductive Medicine, 26(1981) pg. 201-6.
2. Danzer H., Braunstein G.D., et al., “Maternal Serum Human Chorionic Gonadotropic Concentrations and Fetal Sex Predictions.” Fertility and Sterility, 34 (1980) pg. 336-40.
3. Braunstein G.D., et at., “Serum Human Luteinizing Hormone Levels through Normal Pregnancy “, American Journal of Obstetrics and Gynecology, 126 (1976) pg. 678-81.

Complementary Products
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Code: DKO009
Clinical Area:
Incubation: 60+15 min
Sensitivity: N/A
Specificity: N/A
Classification: IVD, CE
Number of Tests: 96
Sample Type: Serum/Plasma
Sample Volume: 20 μL
Assay Range: 5.0 – 200 mIU/mL