Key Features and Values

– Same sample type can be used across all assays to simplify inclusion into routine serology work-up
– Ready to use reagents reduces hands-on time for assay preparation
– Long shelf life cost-effective solution by reducing wastage due to expired kits
– Suitable for inclusion on automated plate systems simplifies scale-up of test volume
– Supported by a complete panel of assays for supporting treatment monitoring of several forms of hormonal dysfunctions

Product Description

TSH ELISA is an immunoenzymatic colorimetric method for the quantitative determination of thyroid-stimulating hormone (TSH, thyrotropin) concentration in human serum or plasma. TSH ELISA kit is intended for laboratory use only.

Scientific Description
Thyroid stimulating hormone (TSH) also referred to as thyrotropin is a glycoprotein synthesised and secreted by the anterior pituitary gland.  It is responsible for the stimulation of the thyroid glands to produce thyroxine (T4) and then Triiodothyronine (T3), which in turn stimulate metabolism of tissues throughout the body1.  It consists of two subunits; the alpha subunit is common to several other hormones including chorionic gonadotrophin, luteinising hormone and follicle stimulating hormone; whereas the beta subunit is specific to TSH1.  Production and secretion of TSH is under the control of thyrotropin releasing hormone (TRH) which is secreted by the hypothalamus to stimulate TSH.  Circulating levels of T3 and T4 also act as a negative feedback on levels of TSH; when T3/T4 levels are elevated, TSH levels are decreased and conversely when T3/T4 levels are low, TSH levels are increased2.
Measurement of serum TSH levels is considered the best screening tool for thyroid dysfunction3 e.g. hypothyroidism or hyperthyroidism.  Typically, in addition to TSH levels, measurements of both T3 and T4 are used in order to support differential diagnosis between primary (linked to the thyroid gland) and secondary (due to the anterior pituitary gland) thyroid disease2.  Co-measurement of serum TSH and free T4 at initial assessment increases diagnostic accuracy for hyperthroidism4.



1. Merck Manual of Diagnosis and Therapy, Thyroid gland disorders
2. Pirahanchi Y, Jialal I. Physiology, Thyroid Stimulating Hormone (TSH) [Updated 2018 Oct 27]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2019 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK499850/
3. Walsh JP.  Managing thyroid disease in general practice.  Med J Aust. 2016 Aug 15;205(4):179-84
4. Kahaly GJ, Bartalena L, Hegedüs L, Leenhardt L, Poppe K, Pearce SH.  2018 European Thyroid Association Guideline for the Management of Graves’ Hyperthyroidism.  Eur Thyroid J. 2018 Aug;7(4):167-186


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Code: DKO013
Clinical Area:
Incubation: 60+20 min
Sensitivity: N/A
Specificity: N/A
Classification: IVD, CE
Number of Tests: 96
Sample Type: Serum/Plasma
Sample Volume: 50 μL
Assay Range: 0.2 – 20 mIU/L