T4 ELISA

Key Features and Values

– Same sample type can be used across all assays to simplify inclusion into routine serology work-up
– Ready to use reagents reduces hands-on time for assay preparation
– Long shelf life cost-effective solution by reducing wastage due to expired kits
– Suitable for inclusion on automated plate systems simplifies scale-up of test volume
– Supported by a complete panel of assays for supporting treatment monitoring of several forms of hormonal dysfunctions

Product Description

Immunoenzymatic colorimetric method for the quantitative determination of thyroxine (T4) concentration in human serum and plasma.  T4 ELISA kit is intended for laboratory use only.

Scientific Description
The thyroid hormone, thyroxine (T4) is produced by the thyroid gland.  An important component in the synthesis is iodine.  The major form of thyroid hormone in the blood is thyroxine (T4).  Thyroxine is converted to the active T3 (three to four times more potent than T4) within cells by deiodinases (5′-iodinase).
Thyroxine-binding globulin (TGB) is the major carrier protein for circulating thyroid hormone.  Only a very small fraction of the circulating hormone is free (unbound) – T4 0.03%.  The thyronines act on the body to increase the basal metabolic rate, affect protein synthesis and increase the body’s sensitivity to catecholamines (such as adrenaline) by permissiveness.  The thyroid hormones are essential to proper development and differentiation of all cells of the human body.  These hormones also regulate protein, fat and carbohydrate metabolism, affecting how human cells use energetic compounds.
Numerous physiological and pathological stimuli influence thyroid hormone synthesis. Thyroid hormones act through an unknown mechanism to inhibit neuronal activity; one of the effects is the reduction of the body temperature. Thyrotoxicosis or hyperthyroidism is the clinical syndrome caused by an excess of circulating free thyroxine, free triiodothyronine, or both.  Both T3 and T4 are used to treat thyroid hormone deficiency (hypothyroidism).
Publications

1. Barker, S.B.,”Determination of Protein Bound Iodine”Journal Biological Chemistry, 173, 175 (1948).
2. Chopra, I.J., Solomon, D.H., an Ho, R.S.,”A Radioimmunoassay of Thyroxine, “J.Clinical EndocrinoL, 33, 865 (1971).
3. Young, D.S., Pestaner, L.C., and Gilberman, U.,”Effects of Drugs on clinical Laboratory Tests.” Clinical Chemistry, 21, 3660 (1975).
4. Sterling, L., Diagnosis and Treatment of Thyroid Disease, Cleveland CRC press, P.19-51 (1975).

Download
Please enter your email address to download the DKO045-T4-IFU.pdf
Code: DKO045
Clinical Area:
Incubation: 60+15 min
Sensitivity: N/A
Specificity: N/A
Classification: IVD, CE
Number of Tests: 96
Sample Type: Serum/Plasma
Sample Volume: 25 μL
Assay Range: 2 – 25 μg/dL