Key Features and Values

– Same sample type can be used across all assays to simplify inclusion into routine serology work-up
– Ready to use reagents reduces hands-on time for assay preparation
– Long shelf life cost-effective solution by reducing wastage due to expired kits
– Suitable for inclusion on automated plate systems simplifies scale-up of test volume
– Supported by a complete panel of assays for supporting treatment monitoring of several forms of hormonal dysfunctions

Product Description

Immunoenzymatic colorimetric method for the quantitative determination of Triiodothyronine (T3) concentration in human serum and plasma. T3 ELISA kit is intended for laboratory use only.

Scientific Description
The triiodothyronine (T3), is a tyrosine-based hormones produced by the thyroid gland.  An important component in the synthesis is iodine.  Thyroxine-binding globulin (TGB) is the major carrier protein for circulating thyroid hormone.  Only the free fraction of T3 is biologically active, a very small fraction of the circulating hormone is unbound – T3 0.3%. The thyronines act on the body to increase the basal metabolic rate, affect protein synthesis and increase the body’s sensitivity to catecholamines (such as adrenaline) by permissiveness.  The thyroid hormones are essential to proper development and differentiation of all cells of the human body. These hormones also regulate protein, fat, and carbohydrate metabolism, affecting how human cells use energetic compounds.  Numerous physiological and pathological stimuli influence thyroid hormone synthesis.
Both excess and deficiency of thyroxine can cause disorders.  Thyrotoxicosis or hyperthyroidism is the clinical syndrome caused by an excess of circulating free thyroxine, free triiodothyronine, or both.  It is a common disorder that affects approximately 2% of women and 0.2% of men.
Hyperthyroidism is the case where there is a deficiency of thyroxine.  Release of T3 from the thyroid gland into the blood is ~5-8 micrograms/day.  In addition, approximately 22 mg/day of T3 is produced by nonthyroidal 5-monodeionination of T4.  T3 has a much faster turnover than T4 (T ~1 day, compared to ~6 days for T4) and has greater biological potency than T4.
Several drugs are known to affect the binding of Triiodothyronine to the thyroid hormone carrier proteins or its metabolism to T3 and complicate the interpretation of T3 results.  Circulating autoantibodies to T3 and hormone-binding inhibitors may interfere.  Measurement of total serum T3 concentrations is a standard and well-validated test of thyroid gland function.

1. Gharib H., et al J. clinical endocrinal.; 33, 509 (1971)
2. Chopra I.J, et al J. Lab Clinical Med., 80 729 (1971)
3.Young D.S., et al Clinical Chemistry, 21 3660 (1975)
4. Sterling L, Diagnosis and Treatment of Thyroid Disease, Cleveland CRC Press, p.9-51 (1975)


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Code: DKO044
Clinical Area:
Incubation: 60+15 min
Analytical range: 50 – 750 ng/dL
Sensitivity: N/A
Specificity: N/A
Classification: IVD, CE
Number of Tests: 96
Sample Type: Serum/Plasma
Sample Volume: 50 μL
Assay Range: 50 – 750 ng/dL