Anti-TPO

Key Features and Values

– Same sample type can be used across all assays to simplify inclusion into routine serology work-up
– Ready to use reagents reduces hands-on time for assay preparation
– Long shelf life cost-effective solution by reducing wastage due to expired kits
– Suitable for inclusion on automated plate systems simplifies scale-up of test volume
– Supported by a complete panel of assays for supporting treatment monitoring of several forms of hormonal dysfunctions

Product Description

Immunoenzymatic colorimetric method for the quantitative determination of Anti-TPO concentration in human serum or plasma. Anti TPO ELISA kit is intended for laboratory use only.

Scientific Description
Anti TPO antibodies (formerly thyroid microsomal antibodies) are directed against a target protein – thyroid peroxidase (TPO) – located in the smooth endoplasmic reticulum of thyroid cells.  Thyroid peroxidase or Thyroperoxidase (TPO) is an enzyme mainly expressed in the thyroid that liberates iodine for addition onto tyrosine residues on thyroglobulin for the production of thyroxine (T4) or triiodothyronine (T3) (thyroid hormones).
It is a frequent epitope of autoantibodies in autoimmune thyroid disease.  In autoimmune disorders, your immune system makes antibodies that mistakenly attack normal tissue such as thyroid peroxidase.  Antibodies that attack the thyroid gland cause inflammation and impaired function of the thyroid.
The presence of anti-TPO antibodies in serum is associated with thyroid autoimmune diseases (Graves’ disease and Hashimoto’s thyroiditis).  Anti-TPO antibodies mostly belong to the IgG class.  Low to moderate levels of serum anti-TPO antibodies can be found in some other autoimmune pathology (eg. systemic lupus erythematosus or Sjogren syndrome) and, rarely, in apparently healthy subjects (especially elderly women).  Anti-TPO antibodies are more sensitive in diagnostics of thyroid autoimmune diseases than antithyroglobulin (anti-TG) antibodies.  However, in some cases anti-TG positive sera may be negative for anti-TPO.  Therefore, combined determination of both types of anti-thyroid antibodies (anti-TPO + anti-TG) provides a more sensitive laboratory diagnostic tool for thyroid autoimmunity.
Publications

1. Volpè R., CRC Press , (1990)
2. Volpè R., Clin. Chem. 40 2132 (1994)
3. Beever K., Clin. Chem. 35 1949-54 (1989)
4. Mat Clin. Chem. 40 2128 (1994)
5. Chiavato L., Autoimmunity 10, 319-31 (1991)
6. Degroot LJ, Thyroperoxidase Thyroid Autoimmunity 207:177 – 182 (1990)

Download
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Code: DKO116
Clinical Area:
Incubation: 30+30+15 min
Sensitivity: N/A
Specificity: N/A
Classification: IVD, CE
Number of Tests: 96
Sample Type: Serum/Plasma
Sample Volume: 10 µL (1:100 predilution)
Assay Range: 5 -320 AU/mL