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Androstenedione ELISA

Key Features and Values
  • Same sample type can be used across all assays to simplify inclusion into routine serology work-up
  • Ready to use reagents reduces hands-on time for assay preparation
  • Long shelf life cost-effective solution by reducing wastage due to expired kits
  • Suitable for inclusion on automated plate systems simplifies scale-up of test volume
  • Supported by a complete panel of assays for supporting treatment monitoring of several forms of hormonal dysfunctions
Product Description

Competitive immunoenzymatic colorimetric method for the quantitative determination of Androstenedione concentration in human serum or plasma. Androstenedione ELISA kit is intended for laboratory use only.

Scientific Description
Androstenedione (also known as Δ4-androstenedione) is a steroid hormone produced in the adrenal glands and the gonads by the action of 17,20-lyase on either DHEA (principle pathway) or 17 a-hydroxyprogesterone (secondary pathway).  Adrenal production of androstenedione is controlled by adrenocorticotrophic hormone (ACTH) and gonadotrophins control production from the gonads.  It is a critical precursor of both the androgen and oestrogen sex hormones acting as an endogenous prohormone; additionally, androstenedione also exhibits weak androgenic activity.  In females, conversion of androstenedione produces approx. 50% of the body’s testosterone and almost all the oestrogen; conversely in males most androstenedione is directly converted to testosterone with little being secreted into the bloodstream.  Measurement of serum androstenedione therefore provides a useful marker of androgen biosynthesis1.
Measurement of androstenedione is useful in several clinical settings: Diagnosis and differential diagnosis of hyperandrogenism, diagnosis of premature adrenarche and diagnosis of congenital adrenal hyperplasia (CAH) and monitoring of CAH therapy.  It has been suggested that measurement of androstenedione levels, together with 17-hydroxyprogesterone and cortisol, constitute the best screening tool for diagnosis of CAH2.  Elevated androstenedione levels have been demonstrated in virilising CAH, polycystic ovary syndrome and may also occur in adrenal and ovarian virilising tumours.
Publications

1. Ballerini MG, Gaido V, Rodríguez ME, Chiesa A, and Ropelato MG.  Prospective and Descriptive Study on Serum Androstenedione Concentration in Healthy Children from Birth until 18 Years of Age and Its Associated Factors.  Dis Markers. 2017; 2017: 9238304
2. Speiser PW, Arlt W, Auchus RJ, Baskin LS, Conway GS, Merke DP, Meyer-Bahlburg HFL, Miller WL, Hassan Murad M, Oberfield SE, White PC.  Congenital Adrenal Hyperplasia Due to Steroid 21-Hydroxylase Deficiency: An Endocrine Society Clinical Practice Guideline.  J Clin Endocrin Metabol, Volume 103, Issue 11, November 2018, Pages 4043–4088

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Code: DKO008
Clinical Area:
Incubation: 60+15 min
Sensitivity: N/A
Specificity: N/A
Classification: IVD, CE
Number of Tests: 96
Sample Type: Serum/Plasma
Sample Volume: 25 μL
Assay Range: 0.1 - 10 ng/mL