Urinary Cortisol ELISA

Key Features and Values

– Same sample type can be used across all assays to simplify inclusion into routine serology work-up
– Ready to use reagents reduces hands-on time for assay preparation
– Long shelf life cost-effective solution by reducing wastage due to expired kits
– Suitable for inclusion on automated plate systems simplifies scale-up of test volume
– Supported by a complete panel of assays for supporting treatment monitoring of several forms of hormonal dysfunctions

Product Description

Competitive immunoenzymatic colorimetric method for the quantitative determination of free Cortisol concentration in Urine. Urinary Cortisol ELISA kit is intended for laboratory use only

Scientific Description
Cortisol is a steroid hormone synthesised from cholesterol in the zona fasciculata of the adrenal cortex.  Approximately 90% of cortisol in plasma is protein-bound to an α2-globulin, cortisol-binding-globulin (CBG), also known as transcortin.  The non-protein bound free cortisol is the biologically active hormone1.
Cortisol secretion from the adrenal gland is mainly controlled by the anterior pituitary hormone, adrenocorticotropic hormone (ACTH).  ACTH is in turn controlled by corticotropin-releasing hormone from the hypothalamus.  Three major factors are involved in the control of the hypothalamic-pituitary-adrenal (HPA) axis and thereby of cortisol: circadian rhythm, negative feedback and stress levels1.  In normal subjects, cortisol levels begin to rise at 3–4 a.m. and reach a peak at 7–9 a.m., with levels falling for the rest of the day2.  Whereas in healthy individual’s late-night levels are undetectable, patients with hyper function of the adrenal lose normal circadian rhythm and have detectable levels of cortisol at midnight3.
Cortisol determinations are used in the assessment of adrenocortical function and other disturbances of the HPA axis.
Measurement of 24-hour urinary free cortisol (UFC) reflects the level of free cortisol within the blood which is filtered by the kidney and excreted in urine; elevated levels are typical in severe cases of hypercortisolism4.  The circadian variation of cortisol level is well documented, and utilisation of a 24-hr urinary collection can account for this and even support identification of endogenous hypercortisolism4.  It is a non-invasive collection method and allows patients to manage collection at home.  Assessment of 24-hr urinary free cortisol levels is a typical first line screening method for Cushing’s syndrome5.  UFC is utilised both for initial diagnosis of Cushing’s syndrome, but may also be used to monitor response to treatment.

 

Publications

1. David W., The Immunoassay Handbook. Third Edition. D.Wild (Ed.) Published by Elsevier Ltd. 2005
2. Rossi GP., Seccia TM. and Pessina AC., ‘Clinical use of laboratory tests for the identification of secondary forms of arterial hypertension’. Crit Rev Clin Sci, 44(1), 2007, pp 1-85
3. Nieman LK., Biller BMK., Findling JW., Newell-Price J., Savage MO., Stewart PM., and Montori VM., ‘The diagnosis of Cushing’s syndrome: an endocrine society clinical practice guideline’. J Clin Endocrinol Metab, 93, 2008, pp 1526-1540
4. Ceccato F, Boscaro M.  Cushing’s Syndrome: Screening and Diagnosis.  High Blood Press Cardiovasc Prev. 2016 Sep;23(3):209-15
5. Luo A, El Gierari ETM, Nally LM, Sturmer LR, Dodd D, Shi RZ.  Clinical utility of an ultrasensitive urinary free cortisol assay by tandem mass spectrometry.  Steroids. 2019 Apr 2;146:65-69

Download
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Code: DKO018
Clinical Area:
Incubation: 60+15 min
Sensitivity: N/A
Specificity: N/A
Classification: IVD, CE
Number of Tests: 96
Sample Type: Urine
Sample Volume: 10 μL
Assay Range: 0.47 - 200 ng/mL