Estradiol ELISA

Key Features and Values
  • Same sample type can be used across all assays to simplify inclusion into routine serology work-up
  • Ready to use reagents reduces hands-on time for assay preparation
  • Long shelf life cost-effective solution by reducing wastage due to expired kits
  • Suitable for inclusion on automated plate systems simplifies scale-up of test volume
  • Supported by a complete panel of assays for supporting treatment monitoring of several forms of hormonal dysfunctions
Product Description

Competitive immunoenzymatic colorimetric method for the quantitative determination of 17β-Estradiol concentration in human serum or plasma. Estradiol ELISA kit is intended for laboratory use only.

Scientific Description
Estradiol (17β-Estradiol) is a sex hormone.  It represents the major estrogen in humans.  Estradiol has not only a critical impact on reproductive and sexual functioning, but also affects other organs including bone structure.  During the reproductive years most Estradiol in women is produced by the ovaries, smaller amounts of Estradiol are also produced by the adrenal cortex.  In men, the testes produce Estradiol.  In plasma Estradiol is largely bound to sex hormone binding globulin (SHBG), also to albumin, only a fraction is free and biologically active.  Serum Estradiol measurement in women primarily reflects the activity of the ovaries.  During pregnancy estrogen levels, including Estradiol, rise steadily towards term.  Estradiol increases due to placental production.  In adult premenopausal women, ovarian production of Estradiol is stimulated by luteinising hormone (LH) and follicle-stimulating hormone (FSH) during the menstrual cycle.
In adult women, Estradiol levels are measured in the evaluation of fertility, menstrual irregularities and to monitor ovarian follicular function during induction of ovulation.  In the female, Estradiol acts as a growth hormone for tissue of the reproductive organs.  The development of secondary sexual characteristics in women is driven by Estradiol.  Estradiol is a regulator of bone maintenance and is involved in male fertility. Post-menopausal women experience an accelerated loss of bone mass due to a reduction in estrogen levels.
Estradiol affects the production of multiple proteins including lipoproteins, binding proteins and proteins responsible for blood clotting.  Estrogens have been found to have neuroprotective function.  Estradiol is also involved in some types of cancer such as breast cancer and cancer of the uterine lining.  In addition there are several benign gynecologic conditions that are dependent on estrogen such as endometriosis, leiomyomata uteri and uterine bleeding.

1. Joshi,U.M., Steroids 34 (1) 35 (1979)
2. D.Exley and R. Abuknesha Febs Letters 91,(2) 162 (1978)
3. Ismail A.A, et al J.Clin.Endocrin.Metab. 34,177-184 (1972)
4. Rajkowski,K.M, et al Steroids 29-5 (1977)
5. Wisdom G.B. Clin. Chem. 22/8, 1243-1255 (1976)
6. D. Sadem, et al J. of Immunolog. Methods 28 125-131(1979)


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Code: DKO003
Clinical Area:
Incubation: 120+30 min
Sensitivity: N/A
Specificity: N/A
Classification: IVD, CE
Number of Tests: 96
Sample Type: Serum/Plasma
Sample Volume: 25 μL
Assay Range: 20 - 2000 pg/mL